Research Highlights
See how top researchers in industry and academia all across the world are using our software tools to make strides in their protein mass spec research.
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ProSight Native
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Proteoform profiling of endogenous single cells from rat hippocampus at scale
P. Su et al. Nat. Biotech. 2025. Online.
May 15, 2025.
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Native Top-down analysis of membrane protein complexes directly from in vitro and native membranes
W. Jung et al. Mol. Cell Prot. 2025, 100993.
May 11, 2025.
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Defining proteoform-specific interactions for drug targeting in a native cell signalling environment
C.A. Lutomski et al. Nat. Chem. 2025, 17, 204-214.
January 13, 2025
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Development of a Novel Label-Free Subunit HILIC-MS Method for Domain-Specific Free Thiol Identification and Quantitation in Therapeutic Monoclonal Antibodies
X. Huang et al. J. Am. Soc. Mass Spectrom. 2024, 35, 3019-3027.
October 30, 2024.
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Hydrophilic Interaction Chromatography Coupled to Ultraviolet Photodissociation Affords Identification, Localization, and Relative Quantitation of Glycans on Intact Glycoproteins
V.K. James et al. J. Prot. Research, 2024, 23, 4684-4693.
September 23, 2024
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ROS-dependent S-palmitoylation activates cleaved and intact gasdermin D
G. Du et al. Nature. 2024, 630:437-446.
April 10, 2024.
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Uniting Native Capillary Electrophoresis and Multistage Ultraviolet Photodissociation Mass Spectrometry for Online Separation and Characterization of Escherichia coli Ribosomal Proteins and Protein Complexes
M.R. Mehaffey et al. Anal. Chem. 2020, 92(22):15202-15211.
November 17, 2020.
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Using 10,000 Fragment Ions to Inform Scoring in Native Top-down Proteomics
A.N. Ives et al. J. Am. Soc. Mass Spectrom. 2020, 31(7):1398-1409.
July 1, 2020.
ProSightPD
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Proteome Discoverer—A Community Enhanced Data Processing Suite for Protein Informatics
B. C. Orsburn. Proteomes. 2021; 9(1): 15. doi: 10.3390/proteomes9010015
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Dissecting ribosomal particles throughout the kingdoms of life using advanced hybrid mass spectrometry methods.
M. V. D. Waterbeemd et al. Nat Commun. 2018; 9(1):2493. doi: 10.1038/s41467-018-04853-x.
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Quantitative top down mass spectrometry identifies proteoforms differentially released during mechanical stimulation of mouse skin.
F. Moehring et al. J Proteome Res. 2018; 17(8):2635-2648. doi: 10.1021/acs.jproteome.8b00109. Epub 2018 Jul 6.
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EThcD and 213 nm UVPD for top-down analysis of bovine seminal plasma proteoforms on electrophoretic and chromatographic time frames. Item
F. P. Gomes et al. Anal Chem. 2020; 92(4):2979-2987. doi: 10.1021/acs.analchem.9b03856. Epub 2020 Jan 29.
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Protamine characterization by top-down proteomics: Boosting proteoform identification with DBSCAN.
G. Arauz-Garofalo et al. Proteomes. 2021; 9(2):21. doi: 10.3390/proteomes9020021.
TDValidator (now integrated into ProSight Native)
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Development of novel methods for non-canonical myeloma protein analysis with an innovative adaptation of immunofixation electrophoresis, native top-down mass spectrometry, and middle-down de novo sequencing
W.I. Deighan et al. Clin. Chem. Lab Med. 2020, 59(4):653-661.
October 20, 2020.
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Accurate Sequence Analysis of a Monoclonal Antibody by Top-Down and Middle-Down Orbitrap Mass Spectrometry Applying Multiple Ion Activation Techniques
Luca Fornelli, Kristina Srzentić, Romain Huguet, Christopher Mullen, Seema Sharma, Vlad Zabrouskov, Ryan T. Fellers, Kenneth R. Durbin, Philip D.Compton, and Neil L. Kelleher
Anal. Chem., 2018; 90, 14, 8421–8429.
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Structural Analysis of Monoclonal Antibodies by Ultrahigh Resolution MALDI In-Source Decay FT-ICR Mass Spectrometry
Yuri E.M. van der Burgt, David P. A. Kilgour, Yury O. Tsybin, Kristina Srzentić, Luca Fornelli, Alain Beck, Manfred Wuhrer, and Simone Nicolardi
Anal. Chem., 2019; 91, 3, 2079–2085.
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Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase
Soo Y. Ro, Luis F. Schachner, Christopher W. Koo, Rahul Purohit, Jonathan P. Remis, Grace E. Kenney, Brandon W. Liauw, Paul M. Thomas, Steven M. Patrie, Neil L. Kelleher, and Amy C. Rosenzweig
Nat Comms., 2019; 10, 2675.